Make reservation

Technical note

Posted

An IPHYS BIF user has lately faced the problem of the depth of field (DoF) for its Optical Projection Tomography. A highly geometrically alternating sample of fascia was blurred for certain projections which compromised the 3D reconstruction. After a little of reversed engineering, calculations, sketching and part delivery. We incorporated the aperture behind the objective to increase the DoF by three fold. If you need some customized solution for your project. That’s what we do! If you have some large objects that needs to be imaged as a whole, for optical clearing and image acquisition, please, contact barbora.radochova@fgu.cas.cz.

Left: The 3D printed holder with a slope of 30 degrees gives 1 mm depth per 2 mm on the axial scale. Right: The change of open and closed aperture for the DoF. Notice the change in sharpness.
Left: The 3D printed holder with a slope of 30 degrees gives 1 mm depth per 2 mm on the axial scale. Right: The change of open and closed aperture for the DoF. Notice the change in sharpness.
After extracting some information from our OPT machine and performing the theoretical calculations for our OPT machine, we simplistically sketched the current tubing and replace it with parts from Thorlabs Inc.
After extracting some information from our OPT machine and performing the theoretical calculations for our OPT machine, we simplistically sketched the current tubing and replace it with parts from Thorlabs Inc.

Back to all news

Czech-BioImaging is a national research infrastructure for biological and medical imaging. It is a distributed infrastructure of leading imaging facilities in the Czech Republic. The infrastructure provides an open access to a wide range of imaging technologies and expertise to all scientists in the Czech Republic and from abroad by a unified and coordinated logistics approach.